ELISA Kit for Immunoglobulin G1 (IgG1)
| Code | Size | Price |
|---|
| CEA074Bo-24T | 24T | £231.00 |
Quantity:
| CEA074Bo-48T | 48T | £382.00 |
Quantity:
| CEA074Bo-96T | 96T | £533.00 |
Quantity:
| CEA074Bo-96T*5 | 96T*5 | £2,293.00 |
Quantity:
| CEA074Bo-96T*10 | 96T*10 | £4,304.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Documents
Further Information
Alternative Names:
IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
Assay Length:
2h
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.
Detection Range:
61.7-5,000ng/mL
Item Name:
Immunoglobulin G1
Method:
Competitive Inhibition
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 29.1ng/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G1 (IgG1).
No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) and analogues was observed.
No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin G1 (IgG1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Immunoglobulin G1 (IgG1) and unlabeled Immunoglobulin G1 (IgG1) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G1 (IgG1). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G1 (IgG1) in the sample.