T7 RNA Polymerase
Product Code: P8870
Product Group: Molecular Biology Enzymes and Master Mixes
Supplier: Abnova Corporation
| Code | Size | Price |
|---|
| P8870-25000Units | 25000 Units | £532.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Applications:
- Functional Study
- In vitro transcription (IVT)
Storage:
Store at -20°C.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Documents
Further Information
Form:
Liquid
Note:
Transcription reaction should be performed under RNase free condition. Use nucleasefree tubes, reagents, and water to avoid RNase contamination. Also, wear gloves when working with RNA.
To obtain optimal condition, NTP concentration can be titrated between 10 ? 15 mM.
The volume of T7 RNA Polymerase can be titrated between 1-2 uL in the IVT reaction to optimize your assay.
To obtain optimal condition, NTP concentration can be titrated between 10 ? 15 mM.
The volume of T7 RNA Polymerase can be titrated between 1-2 uL in the IVT reaction to optimize your assay.
Product Description:
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5? to 3? synthesis of RNA from DNA downstream from its promoter.
Storage Buffer:
In 100 mM Tris-HCl, 20 mM KCl, pH 7.9 (1 mM DTT, 1 mM EDTA, 0.1% Triton? X-100 and 50% (v/v) glycerol)