Inorganic Pyrophosphatase (Yeast)
Product Code: P9370
Product Group: Molecular Biology Enzymes and Master Mixes
Supplier: Abnova Corporation
| Code | Size | Price |
|---|
| P9370-100uL | 100 uL | £195.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Storage:
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Further Information
Concentration:
0.1 U/uL
Form:
Liquid
Product Description:
Inorganic Pyrophosphatase (Yeast) is derived from a recombinant E.Coli strain carrying Saccharomyces cerevisiae ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P2O7-4 + H2O + PPase 2HPO4-2 Inorganic Pyrophosphatase (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
Recommended Dilutions:
In Vitro Transcription
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 50% glycerol