Vaccinia Virus Capping Enzyme
Product Code: P9373
Product Group: Molecular Biology Enzymes and Master Mixes
Supplier: Abnova Corporation
| Code | Size | Price |
|---|
| P9373-50uL | 50 uL | £90.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Storage:
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Further Information
Concentration:
10 U/uL
Form:
Liquid
Product Description:
Vaccinia virus capping enzyme is derived from a recombinant E.coli strain carries the genes for the Vaccinia capping enzyme. This single enzyme is composed of two subunits (D1 and D12) and has three enzymatic activities (RNA triphosphatase and guanylyl transferase by the D1 subunit and guanine methyltransferase by the D12 subunit). Vaccinia virus Capping Enzyme is effective to catalyze the formation of cap structure, which can specifically attach the 7-methylguanylate cap structure (m7Gppp, Cap 0) to the 5' end of RNA. Cap structure (Cap 0) plays an important role in mRNA stabilization, transport, and translation in eukaryotes. Capping RNA by the enzymatic reaction is an effective and simple method which can significantly improve the stability and translation of RNA for in vitro transcription, transfection, and microinjection.
Recommended Dilutions:
In Vitro Transcription
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% glycerol.
Supplied Product:
10x Capping Buffer