CD152 [CTLA-4] (human) (rec.) (untagged)
Code | Size | Price |
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AG-40B-0232-C050 | 50 ug | £190.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: HEK 293 cells
Regulatory Status: RUO
Target Species: Human
Shipping:
BLUE ICE
Storage:
Short term storage:+4°C. Long term storage:-20°C.
Images
Further Information
Alternate Names/Synonyms:
Cytotoxic T-lymphocyte-associated Antigen 4; Cytotoxic T-lymphocyte Protein 4
Biological Activity:
Binds to human CD80.
Concentration:
1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/ug purified protein (LAL test).
Form (Short):
solid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Recombinant protein. Human CD152 [CTLA-4] (aa 36-161) is untagged. Binds to human CD80. Source: HEK 293 cells. Lyophilized. Contains PBS. CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T cell homeostasis and self-tolerance. While CD28 promotes T cell activation and proliferation, CTLA-4 is reported to dampen T cell responses through a variety of mechanisms. Prior to activation, conventional T cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, CTLA-4 has been studied in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.
Molecular Weight:
~18kDa (SDS-PAGE)
NCBI, Uniprot Number:
P16410
Package Type:
Plastic Vial
Product Description:
CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T cell homeostasis and self-tolerance. While CD28 promotes T cell activation and proliferation, CTLA-4 is reported to dampen T cell responses through a variety of mechanisms. Prior to activation, conventional T cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, CTLA-4 has been studied in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.
Purity:
>95% (SDS-PAGE)
Sequence:
Human CD152 [CTLA-4] (aa 36-161) is untagged.
Source / Host:
HEK 293 cells
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C.Working aliquots are stable for up to 3 months when stored at -20°C.