Flagellin (rec.) (untagged) (highly active)

Contact us
AdipoGen Life Sciences
Product Code: AG-40B-0243
Product Group: Other Proteins
CodeSizePrice
AG-40B-0243-C05050 ug£340.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: E.coli
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Shipping:
BLUE ICE
Storage:
Short term storage:+4°C. Long term storage:-20°C.

Images

1 / 1
Flagellin (AG-40B-0243) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0243). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10?g of Flagellin (Prod. No. AG-40B-0243), but no expression of CCL20 and IL-6 could be observed (not shown).
❮❯

Flagellin (AG-40B-0243) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0243). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10?g of Flagellin (Prod. No. AG-40B-0243), but no expression of CCL20 and IL-6 could be observed (not shown).

Documents

Further Information

Alternate Names/Synonyms:
FliC
Biological Activity:
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse.
Concentration:
1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.001EU/ug purified protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Recombinant protein. Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495). Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Source: E. coli. Lyophilized. Contains PBS. Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Molecular Weight:
~54kDa (SDS-PAGE)
NCBI, Uniprot Number:
P06179
Package Type:
Plastic Vial
Product Description:
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Purity:
>95% (SDS-PAGE)
Sequence:
Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).
Source / Host:
E. coli
Specificity:
Binds to human and mouse TLR5 (Toll-like receptor 5).
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C.