BCIP p-toluidine salt

Chemodex
Product Code: CDX-B0024
Supplier: Chemodex
CodeSizePrice
CDX-B0024-M100100 mg£51.00
Quantity:
CDX-B0024-G0011 g£312.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
Short term storage:+20°C. Long term storage:+4°C.

Images

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Chemical Structure

Chemical Structure

Documents

Further Information

Alternate Names/Synonyms:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; X-phosphate p-toluidine salt
Appearance:
Powder.
CAS:
09/06/6578
EClass:
32160000
Form (Short):
solid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChiKey:
QEIFSLUFHRCVQL-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 6578-06-9. Formula: C8H6BrClNO4P . C7H9N. MW: 433.62. BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
MDL:
MFCD00040636
Molecular Formula:
C8H6BrClNO4P . C7H9N
Molecular Weight:
433.62
Package Type:
Vial
Product Description:
BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
Purity:
>95% (HPLC)
SMILES:
BrC1=CC=C(NC=C2OP([O-])(O)=O)C2=C1Cl.[NH3+]C3=CC=C(C)C=C3
Solubility Chemicals:
Soluble in DMSO (25mg/ml) or DMF (25mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) E. Epstein, et al.; Am. J. Clin. Pathol. 48, 530 (1967) | (2) P.L. Wolf, et al.; Experientia 23, 183 (1967) | (3) P.L. Wolf, et al.; Enzymologia 35, 154 (1968) | (4) C.-I. Chang, et al.; Sensors 12, 2710 (2012) | (5) Y. Li, et al.; Anal. Sci. 32, 215 (2016)