Resorufin benzyl ether
| Code | Size | Price |
|---|
| CDX-R0014-M005 | 5 mg | £86.00 |
Quantity:
| CDX-R0014-M010 | 10 mg | £126.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
Short term storage:+4°C. Long term storage:-20°C.
Documents
Further Information
Alternate Names/Synonyms:
Benzyloxyresorufin; 7-Benzyloxyresorufin; O7-Benzylresorufin; 7-Benzyloxy-3H-phenoxazin-3-one
Appearance:
Orange to red powder.
CAS:
87687-02-3
EClass:
32160000
Form (Short):
solid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H315 - H319 - H335
InChi:
InChI=1S/C19H13NO3/c21-14-6-8-16-18(10-14)23-19-11-15(7-9-17(19)20-16)22-12-13-4-2-1-3-5-13/h1-11H,12H2
InChiKey:
XNZRYTITWLGTJS-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 87687-02-3. Formula: C19H13NO3. MW: 303.31. Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 ?M to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=585nm.
MDL:
MFCD00171613
Molecular Formula:
C19H13NO3
Molecular Weight:
303.31
Package Type:
Vial
Precautions:
P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Product Description:
Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 uM to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=585nm.
Purity:
>98% (HPLC)
Signal word:
Warning
SMILES:
O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3N=C2C=C1
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) R.T Mayer, at al.; Biochem. Pharmacol. 40, 1645 (1990) | (2) D.M. Stresser, et al.; Drug Metab. Dispos. 28, 1440 (2000) | (3) A.B. Renwick, et al.; Xenobiotica 31, 861 (2001) | (4) D.M. Stresser, et al.; Drug Metab. Dispos. 30, 845 (2002) | (5) B.M.A. Lussenburg, et at.; Anal. Biochem. 341, 148 (2005) | (6) J.R. Reed, et al.; Biochem. Pharmacol. 95, 126 (2015) | (7) J. Reinen, et al. J. Biomol. Screen. 20, 1246 (2015) | (8) M.J. Traylor, et al.; Insect Biochem. Mol. Biol. 90, 14 (2017) | (9) L. Rutz, et al.; Ach. Toxicol. 94, 4159 (2020)