anti-cAMP, Rabbit Monoclonal (RM466)

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RevMab Biosciences
Product Code: REV-31-1358-00
Product Group: Primary Antibodies
CodeSizePrice
REV-31-1358-00-C05050 ug£484.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM466
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
BLUE ICE
Storage:
Long term storage:-20°C.

Images

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Competitive ELISA showing the specificity of anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 ?l/well). 0.05 ?g/ml of anti-cAMP (RM466) (50 ?l/well) was added and incubated. After wash and block, cAMP and other cyclic nucleotides or nucleoside phosphates (25 ?l/well) were added along with 25 ?l/well of 1/5,000 diluted HRP conjugated cAMP. TMB was used to develop the color after incubation and wash.
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Competitive ELISA showing the specificity of anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 ?l/well). 0.05 ?g/ml of anti-cAMP (RM466) (50 ?l/well) was added and incubated. After wash and block, cAMP and other cyclic nucleotides or nucleoside phosphates (25 ?l/well) were added along with 25 ?l/well of 1/5,000 diluted HRP conjugated cAMP. TMB was used to develop the color after incubation and wash.

Documents

Further Information

Alternate Names/Synonyms:
Cyclic Adenosine Monophosphate
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
KLH-conjugated cAMP.
Long Description:
Recombinant Antibody. RM466 reacts to cAMP (Cyclic Adenosine Monophosphate). No cross-reactivity with other cyclic nucleotide or nucleoside phosphate. Isotype: Rabbit IgG. Immunogen: KLH-conjugated cAMP. Applications: ELISA. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.
Package Type:
Vial
Product Description:
Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.
Purity:
Protein A purified.
Source / Host:
Rabbit
Specificity:
RM466 reacts to cAMP (Cyclic Adenosine Monophosphate). No cross-reactivity with other cyclic nucleotide or nucleoside phosphate.
Transportation:
Non-hazardous
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.