anti-5-Formylcytosines (5-fC), Rabbit Monoclonal (RM477)

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RevMab Biosciences
Product Code: REV-31-1369-00
Product Group: Primary Antibodies
CodeSizePrice
REV-31-1369-00-C05050 ug£592.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM477
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
BLUE ICE
Storage:
Long term storage:-20°C.

Images

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ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
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ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).

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Further Information

Alternate Names/Synonyms:
5-fC
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
KLH-conjugated 5-Formylcytosine.
Long Description:
Recombinant Antibody. RM477 reacts to 5-Formylcytosine (5-fC) in DNA. No cross-reactivity with non-modified cytosine and other modified cytosines in DNA. Isotype: Rabbit IgG. Immunogen: KLH-conjugated 5-Formylcytosine. Applications: ELISA, DotBlot. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. 5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
Package Type:
Vial
Product Description:
5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
Purity:
Protein A purified.
Source / Host:
Rabbit
Specificity:
RM477 reacts to 5-Formylcytosine (5-fC) in DNA. No cross-reactivity with non-modified cytosine and other modified cytosines in DNA.
Transportation:
Non-hazardous
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.