5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair

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RevMab Biosciences
Product Code: REV-31-1111-MK
Product Group: ELISA Kits
CodeSizePrice
REV-31-1111-MK-KI011 Set£760.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Species Independent
Shipping:
BLUE ICE
Storage:
Long term storage:-20°C.

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Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Hydroxymethylcytosine (clone RM236) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
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Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Hydroxymethylcytosine (clone RM236) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.

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Further Information

Alternate Names/Synonyms:
5-hmC
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1) Capture Antibody: Anti-5-Hydroxymethylcytosine Rabbit Monoclonal Antibody, Clone RM236, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Long Description:
Matched Pair Detection Set. This antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Package Type:
Vial
Product Description:
5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system.
Specificity:
This antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Transportation:
Non-hazardous
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.