5-Formylcytosines (5-fC) DNA Matched Antibody Pair

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RevMab Biosciences
Product Code: REV-31-1369-MK
Product Group: ELISA Kits
CodeSizePrice
REV-31-1369-MK-KI011 Set£760.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Species Independent
Shipping:
BLUE ICE
Storage:
Long term storage:-20°C.

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Detection of 5-Formylcytosines (5-fC) in linear denatured DNA (426 bp). Sandwich ELISA using anti-Formylcytosines (5-fC) (clone RM477) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
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Detection of 5-Formylcytosines (5-fC) in linear denatured DNA (426 bp). Sandwich ELISA using anti-Formylcytosines (5-fC) (clone RM477) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.

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Further Information

Alternate Names/Synonyms:
5-fC
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1) Capture Antibody: Anti-5-Formylcytosine (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Long Description:
Matched Pair Detection Set. This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Package Type:
Vial
Product Description:
5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
Specificity:
This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Transportation:
Non-hazardous
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.