rpmB/L28 Antibody

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ProSci
Product Code: PSI-10059
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10059-0.02mg0.02mg£150.00
Quantity:
PSI-10059-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
rpmB/L28 Antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation of rpmB/L28 in E. coli </strong><br> Loading: 15 μg of lysate Antibodies: rpmB/L28 10059, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 WB Validation of rpmB/L28 in E. coli </strong><br> Loading: 15 μg of lysate Antibodies: rpmB/L28 10059, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
Large ribosomal subunit protein bL28, 50S ribosomal protein L28, rpmB
Application Notes:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Background:
The rpmB/L28 protein is a component of the 50S subunit of the ribosome and is required for ribosome assembly. rpmB/L28 interacts with 23S rRNA and crosslinks to L9. L15 and L17 stimulate the interactions of rpmB/L28 with 23S rRNA. rpmB/L28 is phosphorylated at Thr8. rpmB/L28 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity.
Background References:
  • Maguire, B A, and D G Wild. Molecular Microbiology. 1997, 23(2): 237-45.
  • Sk?ld, S E. Biochimie 1981, 63(1): 53-60.
  • Walleczek, J et al. The Journal of biological chemistry 1989, 264(7): 4231-7.
  • Herold, M, and K H Nierhaus. The Journal of biological chemistry 1987, 262(18): 8826-33.
  • Soung, George Y et al. Journal of proteome research 2009, 8(7): 3390-402.
  • Tejedor, F, and J P Ballesta. Biochemistry 1985, 24 (2): 467-72.
Buffer:
rpmB/L28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Klebsiella pneumoniae: (100%), Escherichia fergusonii: (100%), Shigella: (100%)
Immunogen:
Anti-rpmB/L28 antibody (10059) was raised against an 19 amino acid peptide near the carboxy terminus of E. coli rpmB/L28.

The immunogen is located within the last 50 amino acids of rpmB/L28.
NCBI Gene ID #:
946941
NCBI Official Name:
50S ribosomal subunit protein L28
NCBI Official Symbol:
rpmB
NCBI Organism:
Escherichia coli str. K-12 substr. MG1655
Physical State:
Liquid
Predicted Molecular Weight:
Predicted: 9 kD

Observed: 9 kD
Protein Accession #:
P0A7M2.2
Protein GI Number:
67472014
Purification:
rpmB/L28 Antibody is affinity chromatography.
Swissprot #:
P0A7M2
User Note:
Optimal dilutions for each application to be determined by the researcher.