rpmH/L34 Antibody

ProSci
Product Code: PSI-10063
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10063-0.02mg0.02mg£150.00
Quantity:
PSI-10063-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
rpmH/L34 Antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation  of rpmH/L34 in E. coli </strong><br>
Loading: 15 μg of lysate 
Antibodies:  rpmH/L34 10063, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. 
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

<strong>Figure 1 WB Validation  of rpmH/L34 in E. coli </strong><br>
Loading: 15 μg of lysate 
Antibodies:  rpmH/L34 10063, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. 
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
rimA, ssaF, Large ribosomal subunit protein bL34, 50S ribosomal protein L34
Application Notes:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Background:
The rpmH/L34 protein is a component of the 50S subunit of the ribosome. rpmH/L34 can be isolated in a complex with 5S rRNA, tRNA, and other proteins of the large ribosomal subunit. rpmH/L34 is one of six proteins that is completely lost from ribosomes in post-stationary cells. rpmH/L34 was identified as ?antizyme 2?, an inhibitor of the biosynthetic ornithine and arginine decarboxylases, enzymes are involved in the biosynthesis of polyamines.
Background References:
  • Matspalu, E et al. European journal of biochemistry 1982, 124(2): 269-73.
  • Ramagopal, S. European journal of biochemistry 1984, 140(2): 353-61.
  • Heller, J S et al. Proceedings of the National Academy of Sciences of the United States of America 1983, 80(17): 5181-4.
Buffer:
rpmH/L34 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Yersinia: (100%), Shigella: (100%), Salmonella: (100%)
Immunogen:
Anti-rpmH/L34 antibody (10063) was raised against an 17 amino acid peptide near the amino terminus of E. coli rpmH/L34.

The immunogen is located within the first 30 amino acids of rpmH/L34.
NCBI Gene ID #:
948216
NCBI Official Name:
50S ribosomal subunit protein L34?
NCBI Official Symbol:
rpmH
NCBI Organism:
Escherichia coli str. K-12 substr. MG1655
Physical State:
Liquid
Predicted Molecular Weight:
Predicted: 5.4 kD

Observed: 6 kD
Protein Accession #:
P0A7P5.1
Protein GI Number:
67472334
Purification:
rpmH/L34 Antibody is affinity chromatography.
Swissprot #:
P0A7P5
User Note:
Optimal dilutions for each application to be determined by the researcher.