ERBB2/CEN 17 CISH Probe
Product Code: CG0033
Product Group: In-situ Hybridisation Probes (FISH, CISH)
Supplier: Abnova Corporation
| Code | Size | Price |
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| CG0033-100uL | 100 uL | £537.30£597.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Special offer! 10% off Abnova Probes. Read more here. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
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Overview
Regulatory Status: RUO
Target Species: Human
Application: In-Situ Hybridisation
Storage:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Further Information
Antigen Target Species:
Human
Form:
Liquid
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366 or KA6906 ), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366 or KA6906), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (ERBB2 gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 17).
Normal situation:In interphases of normal cells or cells without an amplification involving the ERBB2 gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.
Aberrant situation: In cells with an amplification of the ERBB2 gene region, an increased number of green signals or green signal clusters will be observed.
Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366 or KA6906), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (ERBB2 gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 17).
Normal situation:In interphases of normal cells or cells without an amplification involving the ERBB2 gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.
Aberrant situation: In cells with an amplification of the ERBB2 gene region, an increased number of green signals or green signal clusters will be observed.
Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Product Description:
ERBB2/CEN 17 CISH Probe is designed for the qualitative detection of human ERBB2 gene amplifications as well as the detection of chromosome 17 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommended Dilution:
20x Wash Buffer TBS (WB5) is to be prepared according to the instructions in Assay procedure. All other kit reagents are ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before
Supplied Product:
Reagent Provided:
This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 8p11.1-q11.1 specific for the alpha satellite centromeric region D8Z2 of chromosome 8.
2.Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 17p11.1-q11.1 specific for the alpha satellite centromeric region D17Z1 of chromosome 17.
3. Formamide based hybridization buffer.
This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 8p11.1-q11.1 specific for the alpha satellite centromeric region D8Z2 of chromosome 8.
2.Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 17p11.1-q11.1 specific for the alpha satellite centromeric region D17Z1 of chromosome 17.
3. Formamide based hybridization buffer.