RET Split Probe
Product Code: CS0018
Product Group: In-situ Hybridisation Probes (FISH, CISH)
Supplier: Abnova Corporation
| Code | Size | Price |
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| CS0018-100uL | 100 uL | £537.30£597.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Special offer! 10% off Abnova Probes. Read more here. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
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Overview
Regulatory Status: RUO
Target Species: Human
Application: In-Situ Hybridisation
Storage:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Further Information
Antigen Target Species:
Human
Form:
Liquid
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366 or KA6906 ), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366 or KA6906), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (distal to the RET breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (proximal to the RET breakpoint region).
Normal situation:In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.
Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region.
Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366 or KA6906), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (distal to the RET breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (proximal to the RET breakpoint region).
Normal situation:In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.
Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region.
Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Product Description:
RET Split Probe is designed for the qualitative detection of translocations involving the human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommended Dilution:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:
This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 10q11.21* (chr10:43,687,278-43,856,587) distal to the RET breakpoint region.
2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 10q11.21* (chr10:43,340,888-43,510,171) proximal to the RET breakpoint region.
3. Formamide based hybridization buffer.
This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 10q11.21* (chr10:43,687,278-43,856,587) distal to the RET breakpoint region.
2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 10q11.21* (chr10:43,340,888-43,510,171) proximal to the RET breakpoint region.
3. Formamide based hybridization buffer.