Anti-Mouse CD25 - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C1194-1.0mg | 1.0 mg | £175.00 |
Quantity:
| LEI-C1194-5.0mg | 5.0 mg | £380.00 |
Quantity:
| LEI-C1194-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-C1194-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-C1194-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1 λ
Antibody Clonality: Monoclonal
Antibody Clone: PC61
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Blocking
- Depletion
- Flow Cytometry
- Functional Study
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunoprecipitation (IP)
- In Vivo Assay
- Spatial Biology
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
IL-2-dependent cytolytic mouse T-cell clone B6.1
Long Description:
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
NCBI Gene:
16184
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD25
References
1.) Braley-Mullen, H. et al. (2018) Immunohorizons. 2(1): 54?66. PubMed
2.) Leonard, WJ. et al. (2002) The EMBO Journal 21: 3051
3.) Alt, FW. et al. (1995) Immnnity 3: 521
4.) Greene, WC. et al. (1990) J Invest Dermatol. 94: 27S
5.) Gubin, M. et al. (2018) Cell. 175(4):1014?1030.e19 Journal Link
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