Anti-Human IL-5 Rα - Biotin
| Code | Size | Price |
|---|
| LEI-I-764-50ug | 50 ug | £739.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
Ambient
Storage:
The lyophilized biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Further Information
Conjugate/Tag/Label:
Biotin
Format:
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 um filtered and lyophilized from modified Dulbecco?s phosphate buffered saline (1X PBS) pH 7.2 ? 7.3 containing 50 ug of bovine serum albumin per ug of antibody with no calcium, magnesium, or preservatives present.
Formulation:
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 um filtered and lyophilized from modified Dulbecco?s phosphate buffered saline (1X PBS) pH 7.2 ? 7.3 containing 50 ug of bovine serum albumin per ug of antibody with no calcium, magnesium, or preservatives present.
Immunogen:
Purified Recombinant Human IL-5 Rα (>98%)
Long Description:
The functional IL-5 receptor is a heteromeric complex consisting of an alpha and beta subunit.1 The IL-5Rα chain is exclusively expressed by eosinophils, some basophils and murine B1 cells or B cell precursors.2 Like many other cytokine receptors, alternative splicing of the α-chain gene results in expression of either a membrane bound or soluble form of the α-chain. The soluble form does not lead to signal transduction and therefore has an antagonistic effect on IL-5 signaling. Both monomeric forms of IL-5Rα are low affinity receptors, while dimerization with the β-chain produces a high affinity receptor.3 In either case, the α-chain exclusively binds IL-5 and the intra-cellular portion of IL-5Rα is associated with Janus kinase (JAK) 2, a protein tyrosine-kinase essential in IL-5 signal transduction.4,5 Soluble IL-5Ralpha expression pattern in an eosinophil-associated disease such as nasal polyposis (NP).6
NCBI Gene:
3568
Target:
IL-5 sRα
References
1. Graham, A. et al. (2000) Cytokine 12: 858
2. Coffer, PJ. et al. (2001) Cytokine Growth Factor Rev 12: 19
3. Fiers, W. et al. (1991) Cell 66: 1175
4. Ishikawa, T. et al. (1998) Blood 91: 2264
5. Takatsu, K. et al. (1994) J Exp Med 14: 7404
6. Tavernier, J. et al. (2003) Allergy 58: 371