7-AAD Viability Staining Solution

This solution is stored in PBS, pH 7.2 with 0.09% (w/v) sodium azide at a concentration of 50 ug/ml.

This solution is stored in PBS, pH 7.2 with 0.09% (w/v) sodium azide at a concentration of 50 ug/ml.

7-AAD has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter). 7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.