Anti-Mouse Ly6G Antibody - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-L280-1.0mg | 1.0 mg | £175.00 |
Quantity:
| LEI-L280-5.0mg | 5.0 mg | £380.00 |
Quantity:
| LEI-L280-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-L280-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-L280-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: 1A8
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Depletion
- Flow Cytometry
- Immunohistochemistry- Frozen Section (IHC-F)
- In Vivo Assay
- Mass Cytometry (CyTOF)
- Spatial Biology
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
Ly6G is expressed by neutrophils.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Mouse Ly-6G transfected EL-4J cell line
Long Description:
Ly6G antibody (clone 1A8) recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1,4,5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.
NCBI Gene:
546644
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
Ly-6G
References
1.) Fleming TJ, et al. (1993) J Immunol. 151(5):2399-408
2.) Tsetlin VI. et al. (2015) Trends Pharmacol Sci. 36(2):109-23
3.) Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4.) Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5.) Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6.) Stroncek DF. et al. (2007) Curr Opin Hematol. 14(6):688-93
7.) Wang JX, et al. (2012) Blood. 120(7):1489-1498
8.) Gubin, M. et al. (2018) Cell. 175(4):1014?1030.e19 Journal Link
9.) Lebratti, T.et al. (2021) eLife 10: e65762 Journal Link
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