Anti-Mouse CD115 (CSF-1R) Antibody - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C2169-1.0mg | 1.0 mg | £175.00 |
Quantity:
| LEI-C2169-5.0mg | 5.0 mg | £380.00 |
Quantity:
| LEI-C2169-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-C2169-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-C2169-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: AFS98
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Blocking
- Depletion
- Flow Cytometry
- Functional Study
- In Vivo Assay
- Mass Cytometry (CyTOF)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD115 (CSF-1R) is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid cells, dendritic cells, and osteoclasts.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Not available
Long Description:
CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages1,2. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity3. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer?s disease, and other inflammatory and autoimmune diseases4. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types5,6, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials7.
NCBI Gene:
12978
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD115
References
1. Stanley ER., et al. (1982) Cell. 28:71?81
2. Roussel MF., at al. (1988) Cold Spring Harb Symp Quant Biol. 53:521?530
3. Park-Min, KH., et al. (2020) Exp Mol Med 52, 1239?1254
4. Tak, P. P., at al. (2016) Nat. Rev. Drug Disco. 16, 53?70
5. d?Amore F. (2014) Histopathology. 65:490?500
6. Wei YQ., et al. (2012) PLoS One. 7:e50946
7. R?ttinger D., et al. (2017) J Immunother Cancer. 5(1):53