Anti-Mouse LPAM-1 - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-L307-1.0mg | 1.0 mg | £175.00 |
Quantity:
| LEI-L307-5.0mg | 5.0 mg | £380.00 |
Quantity:
| LEI-L307-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-L307-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-L307-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: DATK32
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Blocking
- Flow Cytometry
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunoprecipitation (IP)
- In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow progenitor cells.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
TK1 cells
Long Description:
LPAM-1 antibody, clone DATK32, recognizes mouse lymphocyte Peyer?s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7). LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells1,2. LPAM-1 mediates adhesion to endothelial cells, promoting leukocyte transmigration across high endothelial venules (HEVs) during the inflammatory response. LPAM-1 binds to several ligands, including vascular adhesion molecule-1 (VCAM-1/CD106), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and fibronectin. MAdCAM-1 is expressed exclusively on endothelial cells of the gut and gut-associated lymphoid tissues, such as Peyer?s patches (PPs), and binding of LPAM-1 to MAdCAM-1 contributes to lymphocyte homing to mucosal tissues3,4. Vedolizumab, an anti-LPAM-1 monoclonal antibody, is approved to treat inflammatory bowel disease and effectively induces and maintains remission in Crohn's disease and ulcerative colitis5-8.
NCBI Gene:
311144
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
LPAM-1
References
1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905
2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26
3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8
4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93
5. Sandborn WJ, et al. (2013) N Engl J Med. 369:711?721
6. Feagan BG, et al. (2013) N Engl J Med. 369:699?710
7. Kopylov U, et al. (2019) Dig Liver Dis. 51:68?74
8. Danese S, et al. (2019) Gastroenterology.157:1007?1018 e1007
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