Anti-Mouse CD273 (PD-L2) - Purified in vivo PLATINUM™ Functional Grade
Code | Size | Price |
---|
LEI-P679-1.0mg | 1.0 mg | £283.00 |
Quantity:
LEI-P679-5.0mg | 5.0 mg | £456.00 |
Quantity:
LEI-P679-25mg | 25 mg | £1,335.00 |
Quantity:
LEI-P679-50mg | 50 mg | £1,938.00 |
Quantity:
LEI-P679-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3.2.B8
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Blocking
- Flow Cytometry
- In Vivo Assay
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Images
Further Information
Antigen Distribution:
PD-L2 is expressed on dendritic cells, liver, few transformed cell lines, and a subset of macrophages.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Made in PD-L2 knockout mouse fusion partner X63-Ag8.653 myeloma cells
Long Description:
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a co-inhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.
NCBI Gene:
80380
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
PD-L2
References
1.) Akbari O, Stock P, Singh AK, Lombardi V, Lee WL, Freeman GJ, Sharpe AH, Umetsu
DT, Dekruyff RH. PD-L1 and PD-L2 modulate airway inflammation and iNKT-cell-
dependent airway hyperreactivity in opposing directions. Mucosal Immunol. 2010; 3:81-
91. PMCID: PMC2845714
2.) . Xiao Y, Yu S, Zhu B, Bedoret D, Bu X, Francisco LM, Hua P, Duke-Cohan JS, Umetsu DT,
Sharpe AH, DeKruyff RH*, Freeman GJ* (* indicates co-senior authors). RGMb is a
novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory
tolerance. J Exp Med. 2014; 211:943-59. PMCID: PMC4010901.
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