Anti-RNase L - Purified in vivo GOLD™ Functional Grade

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Leinco Technologies
Product Code: LEI-R6000
Product Group: Primary Antibodies
CodeSizePrice
LEI-R6000-1mg1 mg£175.00
Quantity:
LEI-R6000-5mg5 mg£380.00
Quantity:
LEI-R6000-25mg25 mg£1,014.00
Quantity:
LEI-R6000-50mg50 mg£1,556.00
Quantity:
LEI-R6000-100mg100 mg£2,159.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 2000000000
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
  • Primate
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • In Vivo Assay
  • Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Purified, full-length, recombinant, human RNaseL produced in insect cells.
Long Description:
RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2?,5?-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2?,5?-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9.
NCBI Gene:
6041
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
RNaseL

References

1. Zhou A, et al. (2005) J Interferon Cytokine Res. 25(10):595-603 2. Silverman RH. (2007) Cytokine Growth Factor Rev. 18(5-6):381-8 3. Wreschner DH, et al. (1981) Nucleic Acids Res. 9(7):1571-81 4. Cooper DA, et al. (2014) Nucleic Acids Res. 42(8):5202-16 5. Castelli JC, et al. (1997) J Exp Med. 186(6):967-72 6. Zhou A, et al (1997) EMBO J. 16(21):6355-63 7. Xiang Y, et al. (2003) Cancer Res. 63(20):6795-801 8. Meyer MS, et al. (2010) Carcinogenesis. 31(9):1597-603 9. Silverman RH. (2003) Biochemistry. 42(7):1805-12