Anti-Human/Mouse CDCP1 (CD318) - Purified in vivo GOLD™ Functional Grade

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Leinco Technologies
Product Code: LEI-C9100
Product Group: Primary Antibodies
CodeSizePrice
LEI-C9100-1.0mg1.0 mg£175.00
Quantity:
LEI-C9100-5.0mg5.0 mg£380.00
Quantity:
LEI-C9100-5mg5.0 mg£456.00
Quantity:
LEI-C9100-25mg25 mg£1,014.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: 9A2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
CDCP1 is widely expressed in the esophagus, skin, colon, duodenum, stomach and other tissues.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
His-tagged recombinant fragment from the internal region of human CUB domain-containing protein 1
Long Description:
CDCP1 is a transmembrane glycoprotein of molecular weight 140 kDa. CDCP1 is a ligand for a receptor molecule (CD6) that is expressed on some T-cells and is thought to be involved in T-cell migration and chemotaxis. It has a large extracellular domain that includes two CUB domains, and a smaller intracellular domain. The smaller intracellular domain contains five tyrosine residues (Y707, Y734, Y743, Y762 and Y806). CDCP1 is cleaved next to Arg368 (at the extracellular domain) by serine proteases. This cleavage yields a truncated molecule with a molecular weight of 80 kDa.1 CDCP1 is not typically cleaved In vivo. However, its cleavage can be induced during tumorigenesis or tissue injury.2 CDCP1 acts as a substrate for Src family kinases which exclusively mediate the phosphorylation of CDCP1. In cultured cells, tyrosine phosphorylation of CDCP1 transpires when cells are stimulated to detach via trypsin or EDTA. This detachment is associated with the phosphorylation of CDCP1 along with the simultaneous dephosphorylation of focal adhesion proteins. Conversely, during cellular attachment, CDCP1 is dephosphorylated, while focal adhesion proteins are simultaneously phosphorylated. Furthermore, CDCP1 is suspected to play a role in autoimmune diseases such as encephalomyelitis, multiple sclerosis and inflammatory arthritis.3
NCBI Gene:
64866
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD318

References

1. Moasser, MM. et al. (2005) Oncogene. 24(34):5333-43. 2. Moasser, MM. et al. (2013) Cancer Res. 73(3):1168-79. 3. Lin, F. et al. (2017) Proc Natl Acad Sci U S A. 114(33):E6912-21.

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