Anti-Human Alpha Defensin-5 (HD5) - Purified in vivo GOLD™ Functional Grade

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Leinco Technologies
Product Code: LEI-D400
Product Group: Primary Antibodies
CodeSizePrice
LEI-D400-1mg1 mg£175.00
Quantity:
LEI-D400-5mg5 mg£380.00
Quantity:
LEI-D400-25mg25 mg£1,014.00
Quantity:
LEI-D400-50mg50 mg£1,556.00
Quantity:
LEI-D400-100mg100 mg£2,159.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: 8C8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • In Vivo Assay
  • Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
HD5 is highly expressed in the secretory granules of Paneth cells of the ileum.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Recombinant Human HD5 (aa 20-94).
Long Description:
The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.3 Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity2, have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.2 In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.1 Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.2 They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.2 HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn?s disease.3 Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2.
NCBI Gene:
1670
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
HD5

References

1. Porter, EM. et al. (1997) Infect Immun. 65(6):2389-95. 2. Ghosh, D. et al. (2002) Nat Immunol. 3(6):583-90. 3. Wehkamp, J. et al. (2019) Proc Natl Acad Sci U S A. 116(9): 3746?3751. 4. Schwaderer, AL. et al. (2012) PLoS One. 7(2):e31712. 5. Hains, D. et al. (2016) US Patent Application Publication No. US 2016/0069905 A1 6. Shen, B. et al. (2005) J Clin Pathol. 58(7):687-94. 7. Ranki, A. et al. (2015) Clin Immunol. 158(2):212-20. 8. Hulscher, JB. et al. (2016) Pediatr Res. 80(2):306-10.