Anti-Human Fibulin-4 - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-F2205-1mg | 1.0 mg | £283.00 |
Quantity:
| LEI-F2205-5mg | 5.0 mg | £456.00 |
Quantity:
| LEI-F2205-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-F2205-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-F2205-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Antibody Clone: 5G11
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- In Vivo Assay
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
Fibulin-4 is predominantly expressed in tissues rich in elastic fibers.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Recombinant, human Fibulin-4 produced in E. coli BL21 cells.
Long Description:
There are currently seven genes in the fibulin family (Fibulin-1, 2, 3, 4, 5, 6, and 7) that are associated with elastic fibers. The fibulins are widely distributed throughout the body and are more prominent in tissues rich in elastic fibers and are often associated with vasculature and elastic tissues. Fibulin-4 appears to be the most critical member of this family of proteins in modulating elastic-fiber formation because studies have shown that fibulin-4-null mice do not produce elastic fibers at all.1 This extracellular glycoprotein has a molecular weight of approximately 49 kD, and is not only considered a structural protein of the extracellular matrix, but also a regulatory factor required for normal elastin expression in human fibroblasts.2 Due to the regulatory role of Fibulin-4, it is thought that a pathophysiological link may exist between the modulation of fibulin-4 levels and diseases, such as WBS (Williams-Beuren syndrome), that are characterized by impaired elastic-fiber formation.1 Furthermore, fibulin-4 has been shown to interact with P53, a crucial homologous protein that prevents cancer formation, and thus functions as a tumor suppressor.3 One study indicated that colon tumors were found to have approximately 2-7-fold increases of fibulin-4 mRNA expression. This suggests that the dysregulated expression of the fibulin-4 gene is associated with human colon tumourigenesis.4
NCBI Gene:
30008
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
Fibulin-4
References
1. DiCorleto, PE. et al. (2009) Biochem J. 423(1): 79?89.
2. Kobayashi, N. et al. (2007) J Biol Chem. 282(16):11805-16.
3. Gallagher, WM. et al. (1999) Oncogene. 18(24):3608-16.
4. Gallagher, WM. et al. (2001) FEBS Lett. 489(1):59-66.