Anti-Human CD64 (FCGR1) - Purified in vivo PLATINUM™ Functional Grade

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Leinco Technologies
Product Code: LEI-I-2015
Product Group: Primary Antibodies
CodeSizePrice
LEI-I-2015-1.0mg1.0 mg£283.00
Quantity:
LEI-I-2015-5.0mg5.0 mg£456.00
Quantity:
LEI-I-2015-25mg25 mg£1,335.00
Quantity:
LEI-I-2015-50mg50 mg£1,938.00
Quantity:
LEI-I-2015-100mg100 mg£2,741.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 10.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Blocking
  • Flow Cytometry
  • Functional Study
  • Immunohistochemistry- Frozen Section (IHC-F)
  • In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
FCGR1 is expressed on monocytes, macrophages, dendritic cells (DCs), and activated granulocytes.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Rheumatoid synovial fluid cells and fibronectin purified human monocytes.
Long Description:
FCGR1 antibody, 10.1, recognizes high-affinity immunoglobulin gamma Fc receptor I (FCGR1), also known as CD64. FCGR1 is a 72 kDa type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells (DCs). FCGR1 can also be induced on neutrophils with IFNγ and G-CSF1. FCGR1 binds with high affinity to monomeric IgG1 and IgG3, and to a lesser extent, IgG42, resulting in phosphorylation of the intracellular FCGR1 ITAM motif and subsequent recruitment of Syk. FCGR1 contributes to inflammation via several mechanisms, including promoting antibody-dependent cell-mediated cytotoxicity (ADCC), clearance of immune complexes, cytokine production, and antigen presentation1,3. CD64-based targeted therapies eliminate M1 pro-inflammatory macrophages and show clinical potential for the treatment of macrophage-mediated chronic inflammatory diseases, such as chronic cutaneous inflammation and rheumatoid arthritis4. In addition, CD64 promotes antitumor responses and mediates cytotoxic killing of tumor cells by macrophages5.
NCBI Gene:
2209
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CD64

References

1. Hulett MD & Hogarth PM. (1998) Mol Immunol. 35(14-15):989-96 2. M. Da?ron., et al. (2009) Blood. 113: 3716?3725 3. Alter G., et al. (2011) Epub. 415(2):160-7 4. Barth S., et al. (2017) Biomedicines. 5(3):56 5. Keler T., et al. (1998) Clin Cancer Res. 4(9):2237-43

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