Anti-Mouse CD370 (CLEC9A/DNGR1) - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-I-2020-1.0mg | 1.0 mg | £283.00 |
Quantity:
| LEI-I-2020-5.0mg | 5.0 mg | £456.00 |
Quantity:
| LEI-I-2020-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-I-2020-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-I-2020-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1F6
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
- In Vivo Assay
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
Long Description:
CLEC9A antibody, 1F6, recognizes C-type lectin domain family 9 member A (CLEC9A), also known as CD370 and DNGR-1. CLEC9A is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. In mice and humans, CLEC9A is expressed by CD8a+ conventional dendritic cells (cDCs) in mice1,2 and BDCA3+ DCs in humans3, which are specialized for the cross-presentation of exogenous antigens on MHC class I4. In mice, type I interferon (IFN)-secreting plasmacytoid DCs (pDCs) also express CLEC9A2. The ligand for CLEC9A is filamentous actin exposed on apoptotic cells5,6, and ligation of CLEC9A results in the cross-presentation and induction of CD8 T cell responses. CLEC9A is a target for vaccine enhancement, and antibodies targeting CLEC9A in vaccination studies lead to enhanced presentation by DCs, eliciting CD8 and CD4 T cell proliferation and strong humoral immunity
NCBI Gene:
232414
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD370
References
1. Murphy KM, et al. (2012) Blood. 119(25):6052?62
2. Ahmet F, et al. (2008) Blood. 112(8):3264-3273
3. Brown GD, et al. (2008) J Biol Chem. 283(24):16693-701
3. Bevan MJ, et al. (2000) J Exp Med. vol. 192 (pg. 1685-1696)
4. Feest C, et al. (2012) Immunity. 36(4):635?45
5. Kitsoulis S, et al. (2012) Immunity. 36(4):646?57
6. Huang Y, et al. (2011) PNAS. 108(6):2384?9
7. Chan CEZ, et al. (2017) 2:31
8. Joffre OP, et al. (2010) Eur J Immunol. 40(5):1255?65
10. Lahoud M., et al. (2015) Eur. J. Immunol. 45:854?864
11. Caminschi I., et al. (2011) J Immunol. 187(2):842-50
12. Reis e Sousa C., et al. (2008) J Clin Invest. 118(6):2098-110