Anti-Mouse CD70 (Clone TAN1-7) - Purified in vivo PLATINUM™ Functional Grade

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Leinco Technologies
Product Code: LEI-I-2025
Product Group: Primary Antibodies
CodeSizePrice
LEI-I-2025-1.0mg1.0 mg£283.00
Quantity:
LEI-I-2025-5.0mg5.0 mg£456.00
Quantity:
LEI-I-2025-25mg25 mg£1,335.00
Quantity:
LEI-I-2025-50mg50 mg£1,938.00
Quantity:
LEI-I-2025-100mg100 mg£2,741.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: TAN1-7
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Functional Study
  • Immunofluorescence (IF)
  • In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
CD70 is expressed on activated T cells, B cells, and dendritic cells.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Recombinant mouse CD70 (extracellular domain, aa residues 41-195)
Long Description:
CD70 antibody, TAN1-7, recognizes CD70, a type II membrane protein and member of the TNF family. CD70 is the ligand for the T cell costimulatory receptor CD27 (also known as TNFRSF27)1 and is expressed on activated T cells, B cells, and dendritic cells (DCs)1,2. Ligation of CD27 on T cells with CD70 on DCs provides costimulatory signals and promotes CD4 and CD8 T cell activation3, differentiation4, and survival5,6. CD70 also regulates B cell activation and antibody production7 and enhances natural killer (NK) cell survival and function8. CD70 is constitutively expressed in several hematological and solid tumors and increases the proliferation and survival of tumor cells and regulatory T cells (Tregs), suggesting it may be an effective therapeutic target9-13. Increased levels of CD70 are also associated with autoimmune disorders, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)14,15.
NCBI Gene:
970
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CD70

References

1. R. A. van Lier., et al. (1997) J. Immunol. 159(10):4959-65 2. R. A. van Lier. et al. (2003) J. Immunol. 170(1):33-40 3. Hintzen, R. Q. et al. (1995) J. Immunol. 154, 2612?2623 4. Soares, H. et al. (2007) J. Exp. Med. 204, 1095?1106 5. Hendriks, J. et al. (2000) Nat. Immunol.1, 433?440 6. Hendriks, J., Xiao, Y. & Borst, J. (2003) J. Exp. Med.198, 1369?1380 7. van Oers MH., et al. (2004) J Immunol. 15;173(6):3901-8. 8. Ochsenbein AF., et al. (2017) 20;130(3):297-309 9. Ansell SM., et al. (2007) Blood. 110:2537?2544 10. Doronina SO., et al. (2006) Cancer Res. 66:2328?2337 11. Weller M., et al. (2002) Cancer Res. 62:2592?2599 12. Held-Feindt, J. & R. Mentlein. (2002) Int. J. Cancer 98:352 13. Chiodi F., et al. (2005) Exp Hematol. 33:1500?1507 14. Han, B. K. et al. (2005) Lupus. 14, 598?606 15. Park, J. K. et al. (2014) Rheumatology. 53, 1896?1900