Anti-Mouse CD178 (FasL) - Purified
Code | Size | Price |
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LEI-M352-500ug | 500 ug | £262.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: MFL3
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
Shipping:
2-8°C
Storage:
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Further Information
Antigen Distribution:
FasL is expressed on activated T cells, NK cells, the eye, and testis.
Concentration:
0.5 mg/ml
Conjugate/Tag/Label:
Purified
Format:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Formulation:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Immunogen:
B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Long Description:
FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells)1-3. Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death4. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites5,6. FasL also contributes to CD8 proliferation and neutrophil recruitment7,8. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling9. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer10. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis11-14.
NCBI Gene:
14103
Target:
CD178
References
1. Okumura K., et al. (1994) Proc Natl Acad Sci USA. 91:4930?4934
2. Nagata S., et al. (1995) J Immunol. 154:3806?3813
3. Saito T., et al. (1995) J Exp Med. 181:1235?1238
4. Ferguson T A., et al. (1995) Science. 270:1189?1192
5. Duke R C., et al. (1995) Nature (London). 377:630?632
6. Fink PJ. (2000) J Immunol. 165(10):5537-43
7. Matsuzawa A., et al. (1998) J Immunol. 161: 4484?4488
8. Nagata S., et al. (1998) Nat Med. 4(1):31-6
9. Hueber AO., et al (2019) Cancers (Basel). 11(5):639
10. Kabelitz D., et al. (2000) Cancer Res. 60: 822?828
11. Giannarelli D., et al. (2000) Int J Cancer. 89: 127?132
12. Kanno H., et al. (2000) Br J Cancer. 82: 1446?1452.
13. Nagano H., et al. (Cancer) Br J Cancer. 82: 1211?1217