Anti-Human CD195 (CCR5) - Purified in vivo GOLD™ Functional Grade

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Leinco Technologies
Product Code: LEI-I-2501
Product Group: Primary Antibodies
CodeSizePrice
LEI-I-2501-1.0mg1.0 mg£175.00
Quantity:
LEI-I-2501-5.0mg5.0 mg£380.00
Quantity:
LEI-I-2501-25mg25 mg£1,014.00
Quantity:
LEI-I-2501-50mg50 mg£1,556.00
Quantity:
LEI-I-2501-100mg100 mg£2,159.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: HEK/1/85a/7a
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
CHO cells transfected with full-length human CD195 (CCR5).
Long Description:
CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1,2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.
NCBI Gene:
1234
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD195

References

1. Mackay CR., et al. (1997) Proc Natl Acad Sci. 94(5):1925-30 2. Doms RW., et al. (1999) Proc Natl Acad Sci. 96(9):5215-5220 3. Parmentier M., et al. (1996) Biochemistry. 35(11):3362-7 4. Berger EA., et al. (1996) Science. 272(5270):1955-8 5. Sodroski J., et al. (1996) Cell. 1996 85(7):1135-48 6. Doms RW., et al. (1996) Cell. 85(7):1149-58 7. Paxton WA., et al. (1996) Nature. 381(6584):667-73 8. Sheppard HW., et al. (1997) Nat Med. 3(10):1160-2 9. Delovitch TL., et al. (2000) J Immunol. 165(2):1102-10 10. Casagrande N., et al. (2020) Cancers (Basel). 12(7):1765