Anti-Mouse CD366 (Tim-3) - Purified (PhenoCycler-Fusion (CODEX)® Ready)
| Code | Size | Price |
|---|
| LEI-T902-50ug | 50 ug | £167.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: RMT3-23
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Spatial Biology
Shipping:
2-8°C
Storage:
This antibody is stable for at least one week when stored at 2-8°C. For long term storage aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Concentration:
0.5 mg/ml
Conjugate/Tag/Label:
Purified (PhenoCycler® Ready)
Format:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Formulation:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Immunogen:
This antibody was produced using recombinant mouse TIM-3
Long Description:
Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.
NCBI Gene:
171285
Target:
Tim-3
References
1. Monney, L. et al. (2002)Nature 415, 536?541.
2. Gao, X. et al. (2012) PLOS ONE 7, e30676.
3. Anderson, A. C. et al. (2007) Science 318, 1141?1143.
4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734?3743.
5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289?2304.
6. Li, X. et al. (2010) Clin. Immunol. 134, 169?177.
7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093?1101.
8. Zhu C, et al.. Nature Immunology. 2005;6:1245?1252.
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