Anti-Mouse CD4 (Clone YTS191) - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C3210-1.0mg | 1.0 mg | £283.00 |
Quantity:
| LEI-C3210-5.0mg | 5.0 mg | £456.00 |
Quantity:
| LEI-C3210-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-C3210-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-C3210-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: YTS191
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Depletion
- Flow Cytometry
- Immunohistochemistry- Frozen Section (IHC-F)
- In Vivo Assay
- Western Blot (WB)
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD4 is expressed on most thymocytes, MHC class II-restricted T cells, a subset of NKT cells, and subsets of dendritic cells and macrophages.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Unknown
Long Description:
CD4 antibody, clone YTS191, recognizes CD4, a 58 kDa type I transmembrane glycoprotein of the Ig superfamily. CD4 is expressed by the majority of thymocytes, MHC class II-restricted T cells (helper T cells and immunosuppressive regulatory T cells), and subsets of natural killer T (NKT) cells, dendritic cells (DCs) and macrophages1-3. On T cells, CD4 is a co-receptor of the T cell receptor (TCR) and interacts with major histocompatibility complex (MHC) II molecules on antigen-presenting cells (APCs). CD4 contributes to T cell development and selection and enhances TCR-dependent signaling by up to 100-fold through the accumulation of Lck4-6. CD4 also contributes to the activation of NKT, macrophages, and DCs7,8.
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CD4
References
1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Esashi E, et al. (2003) J Immunol. 171(6):2773-7
3. Vremec D, et al. (2000) J Immunol. 164(6):2978-86
4. Li QJ, et al. (2004) Nat Immunol. 5:791?9
5. Janeway C. A. Jr. (1992) Annu Rev Immunol. 10:645?74
6. Germain RN. (2002) Nat Rev Immunol. 2(5):309-22
7. Thedrez A, et al. (2007) Blood. 110(1):251-8
8. Gibbings D & Befus AD. (2009) J Leukoc Biol. 86(2):251-9