Leinco Technologies

Anti-Mouse FOXP3-BX024; Alexa Fluor™ 647-RX024

Product Code:
 
LEI-F400
Product Group:
 
Primary Antibodies
Host Type:
 
Rat
Antibody Isotype:
 
IgG2b κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MF-14
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Spatial Biology
  • Western Blot (WB)
Storage:
 
This Phen°Cycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
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LEI-F400-25Tests25 tests£532.00
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Further Information

Antigen Distribution:
FOXP3 is a unique marker of CD4+CD25+ regulatory T cells, distinguishing them from activated CD4+CD25? T cells.
Conjugate/Tag/Label:
AlexaFluor 647, PhenoCycler®
Format:
This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Formulation:
This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Long Description:
FOXP3 is a transcription factor1 required for development of CD4+CD25+ regulatory T cells (Treg) that does not affect CD4+CD25? T cell proliferation2. Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity1. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4+ T cells, and suppresses the transcriptional activities of NFAT and NF-κB3. FOXP3 also inhibits basal activation of PKC-?, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3+CD4+ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity1. FOXP3 is predominantly expressed in CD25+CD4+ Tregs in the thymus and periphery2,4, and abundance increases upon in vitro activation of CD4+CD25+ Tregs2. Additionally, expression of FOXP3 in na?ve T cells can convert these cells to a Treg phenotype4. FOXP3 is virtually undetectable in resting and activated CD4+CD25? T cells2. FOXP3 represents a more specific marker for CD4+ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells4.
NCBI Gene:
50943
Target:
Foxp3

References

1. Wing JB, Tanaka A, Sakaguchi S. Immunity. 50(2):302-316. 2019. 2. Fontenot JD, Gavin MA, Rudensky AY. Nat Immunol. 4(4):330-336. 2003. 3. Bettelli E, Dastrange M, Oukka M. Proc Natl Acad Sci U S A. 2005;102(14):5138-5143. 4. Hori S, Nomura T, Sakaguchi S. Science. 299(5609):1057-1061. 2003. 5. Barber A, Rynda A, Sentman CL. J Immunol. 183(11):6939-6947. 2009. 6. Burzynski LC, Humphry M, Pyrillou K, et al. Immunity. 50(4):1033-1042.e6. 2019. 7. Nakashima H, Hamaguchi Y, Watanabe R, et al. J Immunol. 184(9):4637-4645. 2010. 8. Fallarino F, Volpi C, Zelante T, et al. J Immunol. 183(10):6303-6312. 2009.