Anti-Mouse FOXP3 - Purified (PhenoCycler-Fusion (CODEX)® Ready)
| Code | Size | Price |
|---|
| LEI-F405-25Tests | 25 tests | £456.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: MF-14
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
- Spatial Biology
- Western Blot (WB)
Shipping:
2-8°C
Storage:
This antibody is stable for at least one week when stored at 2-8°C. For long term storage aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
FOXP3 is a unique marker of CD4+CD25+ regulatory T cells, distinguishing them from activated CD4+CD25? T cells.
Concentration:
0.5 mg/ml
Conjugate/Tag/Label:
Purified (PhenoCycler® Ready)
Format:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Formulation:
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Long Description:
FOXP3 is a transcription factor1 required for development of CD4+CD25+ regulatory T cells (Treg) that does not affect CD4+CD25? T cell proliferation2. Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity1. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4+ T cells, and suppresses the transcriptional activities of NFAT and NF-κB3. FOXP3 also inhibits basal activation of PKC-?, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3+CD4+ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity1.
FOXP3 is predominantly expressed in CD25+CD4+ Tregs in the thymus and periphery2,4, and abundance increases upon in vitro activation of CD4+CD25+ Tregs2. Additionally, expression of FOXP3 in na?ve T cells can convert these cells to a Treg phenotype4. FOXP3 is virtually undetectable in resting and activated CD4+CD25? T cells2.
FOXP3 represents a more specific marker for CD4+ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells4.
NCBI Gene:
50943
Target:
Foxp3
References
1. Wing JB, Tanaka A, Sakaguchi S. Immunity. 50(2):302-316. 2019.
2. Fontenot JD, Gavin MA, Rudensky AY. Nat Immunol. 4(4):330-336. 2003.
3. Bettelli E, Dastrange M, Oukka M. Proc Natl Acad Sci U S A. 2005;102(14):5138-5143.
4. Hori S, Nomura T, Sakaguchi S. Science. 299(5609):1057-1061. 2003.
5. Barber A, Rynda A, Sentman CL. J Immunol. 183(11):6939-6947. 2009.
6. Burzynski LC, Humphry M, Pyrillou K, et al. Immunity. 50(4):1033-1042.e6. 2019.
7. Nakashima H, Hamaguchi Y, Watanabe R, et al. J Immunol. 184(9):4637-4645. 2010.
8. Fallarino F, Volpi C, Zelante T, et al. J Immunol. 183(10):6303-6312. 2009.
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