Anti-Rat Kappa Light Chain - Purified in vivo PLATINUM™ Functional Grade

Product Code: LEI-I-2026

Code	Size	Price

LEI-I-2026-1.0mg

1.0 mg

£283.00

Quantity:

LEI-I-2026-5.0mg

5.0 mg

£456.00

Quantity:

LEI-I-2026-25mg

25 mg

£1,335.00

Quantity:

LEI-I-2026-50mg

50 mg

£1,938.00

Quantity:

LEI-I-2026-100mg

100 mg

£2,741.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: Mouse IgG2a k

Antibody Clonality: Monoclonal

Antibody Clone: MAR 18.5

Regulatory Status: RUO

Target Species: Rat

Applications:

ELISpot
Enzyme-Linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
In Vivo Assay
Western Blot (WB)

Shipping:

2-8°C

Storage:

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:

Immunoglobulins consist of heavy chains and light chains. Kappa is a class of light chain and is encoded by the V (variable), J (joining), and C (constant) segments.

Concentration:

? 5.0 mg/ml

Conjugate/Tag/Label:

Purified in vivo Functional Grade, in vivo PLATINUM™

Format:

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Formulation:

Immunogen:

Soluble rat immunoglobulin

Long Description:

MAR 18.5 is a monoclonal antibody directed against rat kappa light chains1. MAR 18.5 was generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by the creation of a B cell hybridoma line via fusion of immune spleen with P3X63Ag8 myeloma cells. MAR 18.5 hybridoma cells secrete an IgG2a kappa monoclonal antibody that strongly binds to protein A. Additionally, MAR 18.5 antibody binds similarly to Ig of RI-1a and RI-1b allotypes. MAR 18.5 antibody can be used in combination with anti-CD19 and anti-CD22 for in vivo B cell depletion in mice2,3. In a study on Fcγ receptor-mediated phagocytosis, MAR 18.5 antibody was used as a secondary cross-linking antibody during stimulation of macrophages grown in medium lacking L cell?conditioned medium (LCM) and treated with chilled supernatant from the rat anti-FcγR 2.4G2 hybridoma4. Additionally, MAR 18.5 antibody has been used for T cell isolation and complement lysis in combination with J11d.2 (anti-heat-stable Ag), 2.43 (anti-CD8), M5/114 (anti-class II), and 2.4G2 (anti-FcR)5.

Purity:

?98% monomer by analytical SEC, >95% by SDS Page

References

1. Lanier LL, Gutman GA, Lewis DE, et al. Hybridoma. 1(2):125-131. 1982. 2. S?w?n P, Lang S, Mandal P, et al. Cell Rep.;14(12):2809-2818. 2016. 3. Keren Z, Naor S, Nussbaum S, et al. Blood. 117(11):3104-3112. 2011. 4. Fitzer-Attas CJ, Lowry M, Crowley MT, et al. J Exp Med. 191(4):669-682. 2000. 5. Hurst SD, Sitterding SM, Ji S, Barrett TA. Proc Natl Acad Sci U S A. 94(8):3920-3925. 1997. 6. Nilsson G, Matsson P, Ahlstedt S. Scand J Immunol. 31(1):53-57. 1990. 7. Elbe-B?rger A, Mommaas AM, Prieschl EE, et al. Immunology. 101(2):242-253. 2000. 8. Zheng Y, Zhou ZZ, Lyttle CR, et al. J Leukoc Biol. 44(1):27-32. 1988. 9. Zhou ZZ, Zheng Y, Steenstra R, et al. Autoimmunity. 3(2):125-134. 1989. 10. Jonsson CA, Carlsten H. Int Immunopharmacol. 3(1):31-37. 2003. 11. Mpandi M, Otten LA, Lavanchy C, et al. J Virol. 77(17):9369-9377. 2003. 12. Reitan SK, Hannestad K. Proc Natl Acad Sci U S A. 99(11):7588-7593. 2002.