Anti-Human/Mouse integrin β7 (Clone FIB21) - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-F118-1.0mg | 1.0 mg | £175.00 |
Quantity:
| LEI-F118-5.0mg | 5.0 mg | £380.00 |
Quantity:
| LEI-F118-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-F118-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-F118-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: FIB21
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Blocking
- Flow Cytometry
- In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
Integrin β7 heterodimer α4/β7 is expressed on peripheral lymphocytes, thymocytes and bone marrow progenitors. Integrin β7 heterodimer αE/β7 is expressed on intestinal IEL, dendritic epidermal T cells, T regulatory cells, CD8+ T cells in lymph nodes and lamina propria.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
TK1 cells
Long Description:
Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation. β7 can pair with CD49d to form the heterodimeric integrin receptor α4β7 or with CD103 to form αEβ7. α4β7 integrin is a lymphocyte receptor for the mucosal vascular addressin MADCAM-11. αEβ7 is an α-I domain-containing integrin that is highly expressed by a variety of leukocyte populations at mucosal sites including dendritic cells, intraepithelial T cells, mast cells, and T regulatory cells.
FIB21 was generated by immunizing Wister rats with TK1 murine T cell lymphoma cells2. Spleen cells were then fused with Sp2/0-Ag14 myeloma cells to create a hybridoma line. FIB21 is known to block adhesion to MADCAM-1 and E-cadherin as well as α4β7 integrin binding to VCAM-1 and fibronectin. FIB21 maps to amino acid resides 176-250, which lie within the region of β7 that specifies MADCAM-1 binding3.
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
Integrin Beta 7
References
1. Berlin C, Berg EL, Briskin MJ, et al. Cell. 74(1):185-195. 1993.
2. Andrew DP, Berlin C, Honda S, et al. J Immunol. 153(9):3847-3861. 1994.
3. Tidswell M, Pachynski R, Wu SW, et al. J Immunol. 159(3):1497-1505. 1997.
4. Meerschaert J, Vrtis RF, Shikama Y, et al. J Immunol. 163(11):6217-6227. 1999.
5. Higgins JM, Cernadas M, Tan K, et al. J Biol Chem. 275(33):25652-25664. 2000.