Anti-Mouse CD183 (Clone CXCR3-173) - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C796-1.0mg | 1.0 mg | £283.00 |
Quantity:
| LEI-C796-5.0mg | 5.0 mg | £456.00 |
Quantity:
| LEI-C796-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-C796-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-C796-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: CXCR3-173
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- In Vivo Assay
- Neutralisation
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CXCL3 is expressed on primary memory phenotype CD4+ and CD8+ T cells, naturally occurring CD4+CD25+ Foxp3+ regulatory T cells, natural killer (NK) T cells, and approximately 25% of NK cells. CXCR3-173 recognizes an epitope of CXCR3 expressed on the surface of activated mouse splenocytes.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Mouse N-terminus of CXCR3
Long Description:
CXCR3 (CD183) is a chemokine receptor that binds to three ligands, CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC), that are induced by IFNγ, -α/β, or other proinflammatory cytokines1. CXCR3 is important for natural killer (NK) cell-dependent priming of CD4+ T cells in lymph nodes2, host responses to infection3, and CD4+ T cell responses to allografts4,5.
CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-376,7. Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels6. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting.
CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases6. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.
NCBI Gene:
12766
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CXCR3
References
1. Tokunaga R, Zhang W, Naseem M, et al. Cancer Treat Rev. 63:40-47. 2018.
2. Martin-Fontecha A, Thomsen LL, Brett S, et al. Nat Immunol. 5: 1260-1265. 2004.
3. Khan IA, MacLean JA, Lee FS, et al. Immunity. 12: 483-494. 2000.
4. Hancock WW, Lu B, Gao W, et al. J Exp Med. 192: 1515-1520. 2000.
5. Hancock WW, Gao W, Csizmadia V, et al. J Exp Med. 193: 975-980. 2001.
6. Uppaluri R, Sheehan KC, Wang L, et al. Transplantation. 86(1):137-147. 2008.
7. Krug A, Uppaluri R, Facchetti F, et al. J Immunol. 169(11):6079-6083. 2002.