Anti-Mouse/Human CD49d (Clone PS/2) - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C798-1.0mg | 1.0 mg | £283.00 |
Quantity:
| LEI-C798-5.0mg | 5.0 mg | £456.00 |
Quantity:
| LEI-C798-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-C798-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-C798-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: PS/2
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Flow Cytometry
- Functional Study
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
P815 DBA/2 murine mastocytoma cells.
Long Description:
Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation. CD49d is a single-pass type I membrane glycoprotein also known as integrin alpha-4 (Uniprot Accession P13612). CD49d is the α4 subunit of integrin heterodimers alpha-4/beta-1 (VLA-4; CD49d/CD29; α4β1 integrin) and alph-4/beta-7 (LPAM-1)1. These integrins act as receptors for fibronectin and VCAM1 (CD106). Integrin alpha-4/beta-7 is also a receptor for MADCAM1.
CD49d is expressed on most lymphocytes, granulocytes, monocytes, and thymocytes. CD49d/CD29 (VLA-4; α4β1) is expressed at high levels on the surface of lymphohematopoietic progenitors and is involved in their development and proliferation. CD49d/CD29 integrin/VCAM-1 interactions facilitate B cell adhesion to stromal cells and enhance B cell activation. In the absence of alpha-4 integrins, pre-B cells fail to transmigrate and proliferate.
PS/2 recognizes murine and human CD49d2. PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0. Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands3. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture2. PS/2 is IgG2b κ.
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CD49D
References
1. Holzmann B, Weissman IL. EMBO J. 8(6):1735-1741. 1989.
2. Miyake K, Weissman IL, Greenberger JS, et al. J Exp Med. 173(3):599-607. 1991.
3. Andrew DP, Berlin C, Honda S, et al. J Immunol. 153(9):3847-3861. 1994.
4. Miyake K, Medina K, Ishihara K, et al. J Cell Biol. 114(3):557-565. 1991.
5. Enghofer M, Bojunga J, Ludwig R, et al. Am J Physiol. 274(5):E928-E935. 1998.
6. Hokibara S, Takamoto M, Isobe M, et al. Clin Exp Immunol. 114(2):236-244. 1998.
7. Fukuoka M, Fukudome K, Yamashita Y, et al. Blood. 96(13):4267-4275. 2000.
8. Omenetti S, Brogi M, Goodman WA, et al. Cell Mol Gastroenterol Hepatol. 1(4):406-419. 2015.
9. Chung KJ, Chatzigeorgiou A, Economopoulou M, et al. Nat Immunol. 18(6):654-664. 2017.
10. Tanneau GM, Hibrand-Saint Oyant L, Chevaleyre CC, et al. J Histochem Cytochem. 47(12):1581-1592. 1999.
11. Tchilian EZ, Owen JJ, Jenkinson EJ. Immunology. 92(3):321-327. 1997.
12. Liu ZJ, Tanaka Y, Fujimoto H, et al. J Immunol. 163(9):4901-4908. 1999.
13. Bellingan GJ, Xu P, Cooksley H, et al. J Exp Med. 196(11):1515-1521. 2002.
14. Bowden RA, Ding ZM, Donnachie EM, et al. Circ Res. 90(5):562-569. 2002.
15. Hirata T, Furie BC, Furie B. J Immunol. 169(8):4307-4313. 2002.
16. Maus UA, Srivastava M, Paton JC, et al. J Immunol. 173(2):1307-1312. 2004.
17. Eshghi S, Vogelezang MG, Hynes RO, et al. J Cell Biol. 177(5):871-880. 2007.
18. Li W, Ishihara K, Yokota T, et al. Glycobiology. 18(1):114-124. 2008.
19. Vaz R, Martins GG, Thorsteinsd?ttir S, et al. Cell Tissue Res. 348(3):569-578. 2012.
20. Zhang Y, Chen YC, Krummel MF, et al. J Immunol. 189(8):3914-3924. 2012.
21. Sens C, Altrock E, Rau K, et al. J Bone Miner Res. 32(1):70-81. 2017.
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