Anti-Human CD18 (ITGB2) - Purified in vivo GOLDTM Functional Grade

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Leinco Technologies
Product Code: LEI-C930
Product Group: Primary Antibodies
CodeSizePrice
LEI-C930-1.0mg1.0 mg£175.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: IB4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunofluorescence (IF)
  • In Vivo Assay
Shipping:
2 - 8°C Wet Ice
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Human peripheral blood lymphocytes
Long Description:
Integrins are a large family of α/β heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation1. The leukocytic cell-adhesion molecule (Leu-CAM) family has three members, LFA-1 (CD18/CD11a), CR3 (CD18/CD11b), and p150,95 (CD18/CD11c), all of which share the β chain CD182. Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody3. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes. For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively. The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems4, 5, 6 and to measure the expression of CD18 in B-ALL cell lines7.
NCBI Gene:
3689
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD18

References

1. Luo BH, Carman CV, Springer TA. Annu Rev Immunol. 25:619-647. 2007. 2. Sanchez-Madrid F, Nagy JA, Robbins E, et al. J Exp Med. 158(6):1785-1803. 1983. 3. Singer II, Kawka DW, DeMartino JA, et al. J Immunol. 150(7):2844-2857. 1993. 4. Hanlon WA, Stolk J, Davies P, et al. J Leukoc Biol. 50(1):43-48. 1991. 5. Seth R, Salcedo R, Patarroyo M, et al. FEBS Lett. 282(1):193-196. 1991. 6. McDonald RJ, St George JA, Pan LC, et al. Inflammation. 17(2):145-151. 1993. 7. Vel?zquez-Avila M, Balandr?n JC, Ram?rez-Ram?rez D, et al. Leukemia. 33(6):1337-1348. 2019.