Anti-Mouse FOXP3 - Dylight® 594

FOXP3 is a unique marker of CD4+CD25+ regulatory T cells, distinguishing them from activated CD4+CD25? T cells.

0.2 mg/ml

DyLight® 650

This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

FOXP3 is a transcription factor1 required for development of CD4+CD25+ regulatory T cells (Treg) that does not affect CD4+CD25? T cell proliferation2. Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity1. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4+ T cells, and suppresses the transcriptional activities of NFAT and NF-κB3. FOXP3 also inhibits basal activation of PKC-?, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3+CD4+ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity1. FOXP3 is predominantly expressed in CD25+CD4+ Tregs in the thymus and periphery2,4, and abundance increases upon in vitro activation of CD4+CD25+ Tregs2. Additionally, expression of FOXP3 in na?ve T cells can convert these cells to a Treg phenotype4. FOXP3 is virtually undetectable in resting and activated CD4+CD25? T cells2. FOXP3 represents a more specific marker for CD4+ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells4.

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Foxp3