Anti-Mouse H-2Db (Clone B22/249) - Purified in vivo GOLD™ Functional Grade
| Code | Size | Price |
|---|
| LEI-B340-1mg | 1.0 mg | £175.00 |
Quantity:
| LEI-B340-5mg | 5.0 mg | £380.00 |
Quantity:
| LEI-B340-25mg | 25 mg | £1,014.00 |
Quantity:
| LEI-B340-50mg | 50 mg | £1,556.00 |
Quantity:
| LEI-B340-100mg | 100 mg | £2,159.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a k
Antibody Clonality: Monoclonal
Antibody Clone: B22/249
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Cytotoxicity
- Depletion
- Immunoprecipitation (IP)
Shipping:
2 - 8°C Wet Ice
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
H-2Db is expressed on the cell surface of lymphocytes.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Mouse H-2Db
Long Description:
H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection2. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1. B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/63. Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays. B22-249.R1 is also known as H-2.m2 in the literature4.
NCBI Gene:
14964
Purity:
?95% by SDS Page, ?95% monomer by analytical SEC
References
1. Yoshida R. Adv Immunol. 124:207-247. 2014.
2. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016.
3. Hammerling GJ, Hammerling U, and Lemke HL. Immunogenetics. 8:433. 1979.
4. Klein J, Huang HJS, Lemke H, et al. Immunogenetics. 8:419. 1979.
5. Maloy WL, H?mmerling G, Nathenson SG, et al. J Immunol Methods. 37(3-4):287-299. 1980.
6. Maloy WL, Coligan JE. Immunogenetics. 16(1):11-22. 1982.
7. Melino M, Nichols E, Strausser H, et al. J lmmunol. 129:222. 1982.
8. Shapiro LH, Dugan ES, Neiderhuber JE. J Exp Med. 162(5):1477-1493. 1985.