Desmin Antibody

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NSJ Bioreagents
Product Code: NSJ-V9390SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9390SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: DES/3255
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Feline (cat)
  • Horse
  • Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Desmin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 8
IHC staining of FFPE human cardiac muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human skeletal muscle with Desmin antibody (clone DES/3255) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human prostate tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE dog smooth muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE cat smooth muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE horse smooth muscle tissue withe Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE cow small intestine tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
8 / 8
SDS-PAGE analysis of purified, BSA-free Desmin antibody (clone DES/3255) as confirmation of integrity and purity.
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IHC staining of FFPE human cardiac muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skeletal muscle with Desmin antibody (clone DES/3255) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE dog smooth muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cat smooth muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse smooth muscle tissue withe Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cow small intestine tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Desmin antibody (clone DES/3255) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the Desmin antibody should be determined by the researcher.
Description:
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human desmin protein was used as the immunogen for the Desmin antibody.
Limitation:
This Desmin antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P17661