Anti-Myc-tag pAb
| Code | Size | Price |
|---|
| MBL-562 | 100 ul | £264.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- ChIP
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:1,000 IP - 2 μL/sample ICC - 1:250
Background:
Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Formulation:
PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 4609 Mouse: 17869
Immunogen Translated:
Peptide synthesis EQKLISEEDL (Myc-tag)-KLH
Reactivity:
This antibody reacts with Myc-tag on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with KLH conjugated synthetic peptide, KLH-EQKLISEEDL (Myc-tag).
Target:
Myc
References
1)
Fukunaka, A., et al., J. Biol. Chem. 286, 16363-16373 (2011)
2)
Shin, M., et al., Development 136, 595-603 (2009)
3)
Nakamura, T., et al., Genes Dev. 22, 1244-1256 (2008)
4)
Nadanaka, S., et al., Mol. Cell Biol. 27, 1027-1043 (2007)
5)
Imamura, M., et al., Hum. Mol. Genet. 14, 775-783 (2005)
6)
Harada, A., et al., J. Biol. Chem. 280, 18418-18424 (2005)
7)
Oinuma, I., et al., J. Neurosci. 24, 11473-11480 (2004)
8)
Naito, M., et al., Mol. Cell Biol. 24, 8418-8427 (2004)
9)
Asada, M., et al., Mol. Cell Biol. 24, 8236-8243 (2004)
10)
Onesto, C., et al., J. Biol. Chem. 279, 34217-34226 (2004)
11)
Fujita, F., et al., Mol. Cell Biol. 23, 7780-7793 (2003)
12)
Oinuma, I., et al., J. Biol. Chem. 278, 25671-25677 (2003)
13)
Uchida, Y., J. Biol. Chem. 278, 366-371 (2003)
14)
Fujita, H., et al., J. Biol. Chem. 277, 45428-45434 (2002)
15)
Vouret-Craviari, V., et al., J. Cell Sci. 115, 2475-2484 (2005)
16)
Iwai, N., et al., Circulation 105, 41-47 (2002)
17)
Kamimoto, T., et al., J. Biol. Chem. 276, 37520-37528 (2001)
18)
Yasui, H., et al., J. Biol. Chem. 276, 15298-15305 (2001)
19)
Inagaki, N., et al., J. Biol. Chem. 275, 27165-27171 (2000)
20)
Yoshizawa, T., et al., Hum. Mol. Genet. 9, 69-78 (2000)
21)
Kondo, T., et al., Mol. Cell Biol. 19, 1136-1143 (1999)
22)
Itoh, M., et al., J. Biol. Chem. 274, 5981-5986 (1999)
23)
Barral, Y., et al., Genes Dev. 13, 176-187 (1999)
24)
Shimomura, T., et al., Mol. Cell Biol. 18, 5485-5491 (1998)