Anti-VSV-G-tag pAb
| Code | Size | Price |
|---|
| MBL-563 | 100 ul | £264.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1:100 (chemiluminescence detection) ICC - 1:100
Background:
Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Formulation:
100 ul volume of PBS containing 50% glycerol/pH 7.2
Immunogen Translated:
YTDIEMNRLGK (VSV-G tag)-KLH
Reactivity:
This antibody reacts with VSV-G-tagged fusion proteins on Western blotting and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using ammonium sulfate precipitation. The rabbit was immunized with KLH-YTDIEMNRLGK
Target:
VSV-G
References
1) Patterson, A. M., et al., Inflamm. Bowel Dis. 18, 1112-1126 (2012)
2) Page, A., et al., J. Invest. Dermatol. 130, 1598-1610 (2010)
3) Smetsers, T. F., et al., Cancer Res. 63, 2965-2970 (2003)
4) Fanning, A. S., et al., FASEB J. 16, 1835-1837 (2002)
5) Nix, S. L., et al., J. Biol. Chem. 275, 41192-41200 (2000)
6) Okada, Y., et al., J. Biol. Chem. 275, 17016-17023 (2000)