Anti-TRAF6 (Mouse) pAb

WB - 1:1,000 (chemiluminescence detection system) IP - 2-4 ug/100-500 uL of cell extract from 5x10⁶ cells

The TRAF-C domain is involved in homotypic and heterotypic aggregation of TRAFs and in interaction of the TNF-receptor superfamily. TRAF6 cDNA has been identified as sequences homologous to the TRAF-C domain of TRAF2 and as binding the cytoplasmic tail of CD40 using the yeast two hybrid system. TRAF6 has a TRAF domain in its carboxyl terminus and has a RING finger domain, a cluster of zinc fingers and a coiled-coil domain. TRAF6 interacts strongly with itself, and weakly with both TRAF2 and TRAF3. Overexpression of TRAF6 activates NF-?B. TRAF6 is likely to be a component of the signaling cascade that starts at the IL-1β receptor and results in the activation of NF-?B.

100 ug IgG in 100 uL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Human: 7189 Mouse: 22034

Recombinant mouse terminal TRAF6 N (1-305 aa)

Anti-TRAF6 antibody reacts with murine TRAF6 on Western blotting and immuno- precipitation. Does not cross-react with other TRAF family, TRAF1, TRAF2, TRAF3, TRAF4 and TRAF5. The antibody detects the 58 kDa TRAF6 on Western blotting using total cell lysate from WR19L, NIH3T3 and L5178Y, and the 69 kDa 6myc-tagged-TRAF6 expressed in 293T cells.

1 year

This antibody is an affinity purified rabbit serum using an affinity column. The rabbit was immunized with the recombinant N-terminal amino acids of mouse TRAF6 (1-305 aa).

TRAF6