Anti-VSV-G-tag pAb-Agarose

IP - 20 uL

Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.

Agarose

430 mg of anti-VSV-G-Tag polyclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL.

YTDIEMNRLGK-CP peptide synthesis (carrier protein)

This antibody recognizes VSV-G-Tag peptide sequence (YTDIEMNRLGK) on Immunoprecipitation.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP-YTDIEMNRLGK.

VSV-G