CoralHue® pFucci Set (G1 Orange (cloning vector) + S/G2/M Green (cloning vector)) - Academic

AM-V9001 pFucci-G1 Orange is a Cloning vector encoding CoralHue ® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). Fucci stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Cdt1 is the DNA replication licensing factor. It accumulates during G1 phase in nuclei, but is degraded during S/G2/M phases by ubiquitin-mediated proteolysis. A part of hCdt1 (30-120) is also degradable in a cell cycle dependent manner. CoralHue ® mKO2 has been generated from dimeric CoralHue ® Kusabira-Orange (KO). CoralHue ® mKO2 has a feature of rapid maturation. AM-V9014 pFucci-S/G2/M Green is a Cloning vector encoding CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). Fucci stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-110) is also degradable in a cell cycle dependent manner. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue® monomeric AG1 (mAG1) has been generated from tetrameric CoralHue® Azami-Green (AG).

pFucci Set (G1 Orange (cloning vector) + S/G2/M Green (cloning vector)) - Academic