CircuLex 14-3-3 Gamma ELISA Kit
| Code | Size | Price |
|---|
| MBL-CY-8082 | 96 Assays | £602.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Alternative Names:
YWHAG 14-3-3γ
Background:
14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. There are seven genes, β, γ, ε, ?, ζ, ? and ?, that encode 14-3-3s in most mammals. 14-3-3 proteins have the ability to bind phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, on a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. The family dynamically regulates the activity of target proteins in various signaling pathways that control diverse physiological and pathological processes. More than 100 signaling proteins have been reported as 14-3-3 proteins? targets.
Either an abnormal state of 14-3-3 protein expression or dysregulation of 14-3-3/terget protein interactions contributes to the development of many human diseases. Clinical investigations have demonstrated a correlation between up-regulated 14-3-3 protein levels and poor survival of cancer patients. Several studies have also suggested that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes. Especially, elevated amounts of the gamma isoform (14-3-3 Gamma) are found in the cerebrospinal fluid (CSF) of Creutzfeldt-Jakob disease (CJD) patients and could be as a specific marker of the disease as well as tau protein level.
Description:
The CycLex Research Product CircuLex 14-3-3 Gamma ELISA kit is used for the quantitative measurement of 14-3-3 Gamma (gamma isoform protein) in CSF (Cerebrospinal fluid), cell lysates and other biological samples in units of concentration.
Gene IDs:
Human: 7532 Mouse: 22628
Kit Components:
Microplate, 10X Wash Buffer, Sample/Standard Dilution Buffer, 14-3-3 Gamma Standard, 100X 14-3-3 Gamma Detection Antibody, Detection Antibody Dilution Buffer, 100X HRP conjugated Anti-IgG Antibody, Conjugate Dilution Buffer, Substrate Reagent, Stop Solution
Measurement Range:
Dilution factors need to be taken into consideration in calculating the units of 14to3to3 Gamma concentration. Results exceeding 14to3to3 Gamma level of 16,000 AU /mL should be repeated with diluted samples.
Sensitivity:
better than 250 AU/mL of sample.
References
1. The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. Hsich G, Kenney K, Gibbs CJ, Lee KH, Harrington MG. (1996) N Engl J Med. 335(13):924-30.
2. Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. Wiltfang J, Otto M, Baxter HC, Bodemer M, Steinacker P, Bahn E, Zerr I, Kornhuber J, Kretzschmar HA, Poser S, R?ther E, Aitken A. (1999) J Neurochem. 73(6): 2485-90.
3. An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients. Kenney K, Brechtel C, Takahashi H, Kurohara K, Anderson P, Gibbs CJ Jr. (2000) Ann Neurol. 48(3):395-8.
4. Determination of 14-3-3 protein levels in cerebrospinal fluid from Creutzfeldt-Jakob patients by a highly sensitive capture assay. Peoc'h K, Schr?der HC, Laplanche J, Ramljak S, M?ller WE. (2001) Neurosci Lett. 301(3):167-70.
5. CSF tests in the differential diagnosis of Creutzfeldt-Jakob disease. Sanchez-Juan P, Green A, Ladogana A, Cuadrado-Corrales N, S?anchez-Valle R, Mitrov?a E, Stoeck K, Sklaviadis T, Kulczycki J, Hess K, Bodemer M, Slivarichov? D, Saiz A, Calero M, Ingrosso L, Knight R, Janssens AC, van Duijn CM, Zerr I. (2006) Neurology. 67(4):637-43.
6. Total tau protein in cerebrospinal fluid and diffusion-weighted MRI as an early diagnostic marker for Creutzfeldt-Jakob disease. Satoh K, Shirabe S, Tsujino A, Eguchi H, Motomura M, Honda H, Tomita I, Satoh A, Tsujihata M, Matsuo H, Nakagawa M, Eguchi K. (2007) Dement Geriatr Cogn Disord. 24(3):207-12. (Epub 2007 Aug 10.)
7. The role of cerebrospinal fluid proteins as early diagnostic markers for sporadic Creutzfeldt-Jakob disease. Pennington C, Chohan G, Mackenzie J, Andrews M, Will R, Knight R, Green A. (2009)Neurosci Lett. 455(1):56-9. (Epub 2009 Mar 5.)
8. High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease. Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Mutsukura K, Satoh A, Kataoka Y, Nishida N. (2011) BMC Neurol. 11: 120.